杜仲抗HIV gp41活性部位的研究
EXPERIMENTAL STUDY ON ANTI - HIV ACTIVITY OF
目的：研究杜仲活性成分对HIV gp41六股螺旋束的抑制作用，为寻求新的抗HIV病毒进入抑制剂提供实验依据。 方法：杜仲萃取物配制成1mg/ml的样品液，用高效液相法检测样品对HIV gp41六股螺旋束结构形成的抑制作用，并采用酚试剂法检测样品中的鞣酸含量。 结果：杜仲提取物浓度1mg/ml可有效降低gp41峰高，1mg/ml的样品中含鞣酸10ug/ml 结论：杜仲可抑制HIV gp41六股螺旋束的形成，其有效成份可能不包括鞣酸。
Objective:Investigation the anti - HIV effects of Eucommia ulmoides Oliv, so as to provide experimental basis for searching a new efficacious drug for treatment of AIDS. Methods:Using phytochemistry to isolate compounds from Eucommia ulmoides Oliv, the inhibitory activity of Samples on the gp41 six-helix bundle formation was determined by a modified HPLC. Results:The Samples from Eucommia ulmoides Oliv had potent inhibitory activity on the gp41 six-helix bundle formation. Conclusion:Eucommia ulmoides Oliv inhibits HIV by targeting gp41 six-helix bundle formation.
吴曙光、吕琳
基础医学药学生物化学
HIV gp41病毒进入抑制剂杜仲HPLC法
HIV-1 gp41HIV entry inhibitor agentsEucommia ulmoides Olivsandwich ELISA
吴曙光,吕琳.杜仲抗HIV gp41活性部位的研究[EB/OL].(2010-02-04)[2025-04-26].http://www.paper.edu.cn/releasepaper/content/201002-208.点此复制
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