不同发酵温度对食窦魏斯氏菌发酵酸面团性质及相应面包风味的影响
Effect of different fermentation temperatures on the sourdough and bread aroma properties using Weissella cibaria strain 59
发酵温度对乳酸菌发酵产品的风味物质影响很大,本研究以实验室筛选食窦魏斯氏菌(W)为研究对象,以酸面团常用乳酸菌旧金山乳杆菌(J)作为对照菌株,研究了在10℃、20℃、30℃三种不同温度下发酵酸面团的性质及其对面包风味的影响。结果显示,10℃、20℃低温条件下酸面团发酵值(Fermentation Quotient即FQ) 与30℃传统发酵温度酸面团相近,面包酸味柔和,但低温发酵酸面团还原糖含量、游离氨基酸含量均高于传统发酵温度酸面团。通过GC-MS测定挥发性风味物质分析得到,低温发酵酸面团不仅增加了面包风味物质的种类,出现了正庚醛、2-甲基丁基乙酸酯、反-2-辛烯醛、1-庚醇、桃醛等新的风味物质,而且对面包香气有重要贡献的风味物质含量也高于30℃传统温度下发酵酸面团面包以及对照组。W和J相比较,在10℃时W产酸能力更强,代谢产生还原糖和游离酸含量也更多,其面包风味也更丰富,表现出对低温有更强的适应能力。
Fermentation temperature has great influence on volatile flavor compounds of lactic acid fermentation product. In this study, the sourdough and bread aroma properties fermented with Weissella cibaria strain 59 and San Francisco Lactobacillus were compared at different fermentation temperatures. The results showed that the fermentation quotient was comparative with those in sourdoughs at traditional fermentation temperatures (30℃), but a high reducing sugar and total free amino acids content were noticed. Volatile compounds content in the fermented sourdough breads was also impacted by fermentation temperature. Specific substances such as heptanal, 1-butanol-2-methyl-acetate, 5-heptyldihydro-2(3H)-furanone, (E)-2-Octenal, 1-heptanol were only detected in the sourdough breads under low-temperature fermentation using solid phase micro-extraction/gas chromatography-mass spectrometry (SPME/GC-MS) analysis. Meanwhile, the amount of the important volatile flavor compounds in sourdoughs at low fermentation temperatures (10, 20℃) were higher than the others. In comparison with J, W have stronger acidogenicity and could produce more reducing sugar and total free amino acids content, resulting in a richer flavor. Therefore, W showed stronger adaptability to low temperature.
徐丹、徐学明、张印、吴凤凤、赵迪
微生物学生物化学生物工程学
食窦魏斯氏菌,低温发酵,酸面团,挥发性风味物质
Weissella cibariaLow-temperature fermentationSourdoughvolatile flavor compounds
徐丹,徐学明,张印,吴凤凤,赵迪.不同发酵温度对食窦魏斯氏菌发酵酸面团性质及相应面包风味的影响[EB/OL].(2015-12-30)[2025-08-06].http://www.paper.edu.cn/releasepaper/content/201512-1390.点此复制
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