FoxE1基因启动子甲基化在结直肠癌和正常结直肠粘膜中的比较研究

Objective:To explore the relationship between methylation of FOXE1 gene promotor and tumorigenesis of colorectal cancer.Methods: This study assessed the methylation of FOXE1 on 19 specimen of stage I colorectal cancer and 19 paired specimen of normal colorectal mucosa using realtime quantitative methylation-specific PCR, compared the difference on methylation of FoxE1 between normal colorectal mucosa and colorectal cancer, and explored the relationship between methylation of FOXE1 gene promotor and the clinicopathological features of colorectal cancer.Results: The positive rate of FOXE1 methylation is 63.2%（12/19）in stage I colorectal cancer, while none of the samples from normal colorectal mucosa was positive. The level of FOXE1 methylation of stage I colorectal cancer is significantly higher than that of normal colorectal mucosa (p=0.029). There are no significant correlation between the status of the FOXE1 gene promotor and patient gender, age, location of tumor, histological type, degree of differentiation, invasive depth or vascular invasion.Conclusion: The level of FOXE1 methylation of stage I colorectal cancer is significantly higher than that of normal colorectal mucosa. FoxE1 gene promotor methylation is cance specific in colorectal cancer.