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肝细胞癌中共刺激分子B7-H4表达的临床病理学意义及预后价值

he clinicopathologic signficance and prognostic value of costimulatory molecule B7-H4 expression in human hepatocellular carcinoma

中文摘要英文摘要

目的:观察B7-H4蛋白在肝癌组织和癌旁肝组织中的表达及其差异性,分析B7-H4蛋白在癌旁肝组织表达水平与肝癌患者临床病理特征以及术后生存和复发转移的相关性。方法:应用免疫组化技术对由232例肝癌患者手术切除标本构建的组织芯片进行染色,对芯片的每点在×200下拍照,利用Image-Pro Plus软件进行光密度分析,统计分析B7-H4的差异表达及癌旁B7-H4表达水平与患者总体生存率和复发转移率的相关性。结果:肝癌的癌旁组织B7-H4表达显著高于癌组织(P<0.001)。癌旁B7-H4的表达与乙肝病史(P=0.016)、肿瘤大小(P=0.004)、肿瘤包膜(P=0.006)、血管侵犯(P=0.012)、TNM分期(P=0.002)等临床病理因素存在显著相关性。单因素分析发现,癌旁肝组织B7-H4高表达患者,总体生存率明显低于低表达患者,其复发转移率更高。多因素结果提示,癌旁B7-H4高表达是影响术后生存和复发转移的独立危险因素,癌旁B7-H4高表达组的复发风险是低表达组的近2倍(HR=1.832,95%CI:1.25-2.68,P=0.002)。结论:癌旁B7-H4的高表达是预测病人生存时间及复发的独立危险因素。

Objective: To detect the expression of B7-H4 protein in human hepatocellular carcinoma (HCC) tumor tissues and corresponding peritumor liver tissues, and to analyze the relationship of peritumor B7-H4 expression with patient's clinicopathologic features and postoperative survival and recurrence. Methods: Tumor tissue microarrays containing 232 HCC patients who underwent curative resection between 2002 and 2006 were used to detect the expression of B7-H4. Correlation of peritumor B7-H4 expression with clinicopathologic characteristics for these patients was evaluated. The prognostic significance of peritumor B7-H4 expression was assessed using Kaplan-Meier estimates and Cox proportional hazard model. Results: In HCC, peritumor liver tissues had a significantly higher expression of B7-H4 than matched tumor tissues (P<0.001). Increased peritumor B7-H4 expression significantly and positively correlated with the presence of HBV history (P=0.016), large tumor size (P=0.004), absence of tumor capsule (P=0.006), presence of vascular invasion (P=0.012) and advanced TNM stage (P=0.002). Univariate analyses indicated that high peritumor B7-H4 expression was a significant predictor for reduced overall survival (P<0.001) and recurrence-free survival (P=0.001). Importantly, Cox multivariate analysis authenticated that peritumor B7-H4 expression is an independent index for postoperative survival and recurrence. In particular, HCC patients with high peritumor B7-H4 expression suffered from a risk of recurrence about two times than patients with low peritumor B7-H4 expression (HR=1.832,95%CI:1.25-2.68,P=0.002). Conclusion: Peritumor B7-H4 expression is an independent predictor for postoperative patient survival and tumor recurrence in HCC.

王晓颖、高强

肿瘤学临床医学基础医学

肝细胞癌预后B7-H4免疫组化

Hepatocellular carcinomaPrognosisB7-H4Immunohistochemistery

王晓颖,高强.肝细胞癌中共刺激分子B7-H4表达的临床病理学意义及预后价值[EB/OL].(2013-01-17)[2025-08-18].http://www.paper.edu.cn/releasepaper/content/201301-769.点此复制

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