GbRvd超表达载体的构建及超表达拟南芥的抗病性分析

In this study, Gateway technology was used to construct the overexpression vector of GbRvd, and the PCR detection and sequencing showed that the vector was constructed successfully. The overexpression vector was transferred into the Agrobacterium tumefaciens by using the method of freezing and thawing, transformation of Arabidopsis thaliana was mediated by Agrobacterium tumefacienand. Fifteen transgenic positive plants were obtained through the screening of the kanamycin and PCR detection. The resistance of Verticillium wilt for three independent T3 transgenic lines was analyzed by soil drenching methods, identification results showed that GbRvd overexpression significantly increased the resistance of Arabidopsis thaliana to Verticillium wilt, which further proved that GbRvd could mediate resistance to the disease as an important factor.