二氧化锰纳米酶作为载体递送反义寡核苷酸的研究
Manganese dioxide nanozymes as carriers for antisense oligonucleotides delivery
本文制备了氨基化糖原和牛血清白蛋白修饰的二氧化锰纳米酶，并探究了其作为反义寡核苷酸（Smyd3-ASO）递送载体的可行性和有效性。首先制备了牛血清白蛋白修饰的二氧化锰和氨基化糖原，并将二者交联合成具有正电性的纳米酶载体（NGBM）。NGBM通过静电作用装载Smyd3-ASO。实验结果表明NGBM具有谷胱甘肽氧化酶（GSH-OXD）和过氧化物酶（POD）活性，且具有较高的Smyd3-ASO装载量。流式分析结果表明NGBM可通过巨胞饮途径显著提高Smyd3-ASO的细胞摄取；细胞毒性实验结果表明NGBM/Smyd3-ASO可有效抑制肿瘤细胞生长。
In this study, amino glycogen and bovine serum albumin functionalized manganese dioxide nanozyme was prepared and itsfeasibility and effectiveness as vector for antisense oligonucleotides (Smyd3-ASO) deliverywas investigated. BSA functionalized manganese dioxide andaminated glycogen were synthesized, respectively, and then crosslinked to prepare the positive nanozyme carrier (NGBM). Smyd3-ASO was loaded on NGBMthrough electrostatic interactions. The results showed that NGBMexhibited glutathione oxidase (GSH-OXD) and peroxidase (POD) activities, and had a high Smyd3-ASOloading capacity. Flow cytometry analysis showed that NGBM could significantly improve the cellular uptake of Smyd3-ASO mainlyby macropinocytosis. Cytotoxicity assay showed that NGBM/Smyd3-ASO could effectively inhibit tumor cells growth.
张嘉文、方久锐、张慧杰、陈敬华、马可可
基础医学生物化学生物工程学
药剂学二氧化锰纳米酶反义寡核苷酸纳米载体基因递送
Pharmaceuticsmanganese dioxidenanozymeantisense oligonucleotidesnanocarriergene delivery
张嘉文,方久锐,张慧杰,陈敬华,马可可.二氧化锰纳米酶作为载体递送反义寡核苷酸的研究[EB/OL].(2023-04-04)[2025-08-04].http://www.paper.edu.cn/releasepaper/content/202304-38.点此复制
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