微小RNA-519C-5P在食管癌中表达及其功能研究

In Objectives ：This research was undertaken to explore the expression pattern of miR-519c-5p in esophageal cancer and noncancerous paired specimens, the effect of miR-519c-5p on the EC-109 esophageal cancer cells. Methods ：We conducted RT-PCR to quantify the expression of miR-519c-5p in esophageal cancer and noncancerous paired specimens. Functional assays were respectively employed by proliferation, cell cycle assays. Results: The relative expression of miR-519c-5p in esophageal cancer samples was 4.547122±5.313108 which was significantly higher than 1 of adjacent normal ones (P <0.05). MTT assay revealed that miR-519c-5p mimics significantly promotes proliferation of tumor cell at 48 h after transfection at the concentration of 50 nmol/l; Esophageal cancer cells in G0/G1 stage were significantly decreased, cells in S stage were greatly increased after the treatment with 50 nmol/L miR-519c-5p mimics for 48 h in comparsion with control, P<0.05; For cells in G2/M stage, no significant difference was noted, P>0.05. Conclusions：miR-519c-5p is aberrantly expressed in esophageal cancer, esophageal tumor specimens show increased miR-519c-5p expression compared to their normal counterparts. As miR-19c-5p promotes cell proliferation and esophageal cancer cells in G0/G1 stage, miR-19c-5p might act as oncogene in esophageal cancer.