叶柄粘球菌STXZ77菌株抗肿瘤活性物质的初步分离

In this study, the antitumor activity of strain STXZ77 was studied by toxicity test. The supernatant of the fermentation broth had a highly effective toxicity effect on B16 and HCT-16 cells in dose-manner. The fermentation supernatant lost antitumor activity in more than 60℃ water-bath heating for half an hour, indicated that the main active substance in the fermentation supernatant with thermal instability. the antitumor activity of precipitation from the fermentation supernatant protein by solid ammonium sulfate fractionation was tested. The results show interval of 40% ~ 60% saturation has the strongest cytotoxicity, while interval of 80% ~ 100% weakest. The protein crude extract was obtained by 80% saturation ammonium sulfate precipitation, and then the antitumor active compound was separated from the crude extract by anion-exchange chromatography, which laid an important foundation for the search of new antitumor drugs.