miR-17抑制原代细胞衰老的研究
miR-17 inhibites the senescence of primary fibroblasts
目的：分离培养原代包皮成纤维细胞HFF，研究miR-17对HFF细胞衰老的影响。方法：利用胰酶消化法分离培养HFF；将miR-17利用慢病毒包装系统感染HFF细胞后，qRT-PCR确定感染效率，利用CCK-8研究miR-17对HFF细胞增殖的影响，衰老相关 β-半乳糖苷酶染色观察miR-17对细胞衰老的影响。结果：成功分离得到HFF细胞。CCK-8及衰老相关 β-半乳糖苷酶染色发现，与对照HFF-NC细胞相比，HFF-miR17细胞增殖能力明显升高，β-半乳糖苷酶染色阳性率显著降低。结论：miR-17可促进原代包皮成纤维细胞的增殖，抑制原代细胞的衰老。
Objective：To isolate the human foreskin fibroblasts (HFF), and to study the effect of miR-17 on senescence of HFFs. Methods：Recombinant lentivirus expressing miR-17 infected HFF and miR-17 expression was comfirmed by qRT-PCR. The proliferation and senescence of the HFF were detected by CCK-8 and SA-β-Gal kit, respectively. Results：HFF cells were successfully isolated. Compared with HFF-NC, the proliferation ability of HFF-miR-17 cells was obviously increased while the expression of SA-β-Gal in senescent cells decreased. Conclusion：miR-17 promotes cell proliferation and inhibits cell senescence.
赵蔚明、陈燕、张魏芳
基础医学细胞生物学分子生物学
人包皮成纤维细胞miR-17衰老
Human foreskin fibroblastsmiR-17Senescence
赵蔚明,陈燕,张魏芳.miR-17抑制原代细胞衰老的研究[EB/OL].(2014-12-18)[2025-08-02].http://www.paper.edu.cn/releasepaper/content/201412-540.点此复制
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