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西藏虎头兰高效植株再生体系的研究

中文摘要英文摘要

西藏虎头兰(Cymbidium tracyanum)为兰科兰属多年生草本植物,是一种极具观赏价值的野生花卉,目前关于西藏 虎头兰组培快繁的研究报道甚少。该研究以野生西藏虎头兰种子为外植体,通过分析不同基本培养基和植物激素配比对原球茎诱导、增殖和分化的影响,以及光照时间和培养温度对试管苗生长的影响,筛选出适宜西藏虎头兰植株高效再生的条件。 结果表明:适宜西藏虎头兰生长的基本培养基为 1/2 MS;种子萌发和原球茎诱导的最适培养基为 1/2 MS+1.0 mg·L-1 6-BA +0.5 mg·L-1 NAA,培养 50 d 后,有 95.00%的种子发育成原球茎;原球茎增殖的最适培养基为 1/2 MS+2.0 mg·L-1 NAA, 培养 30 d,增殖倍数为 4.25;原球茎的最适分化培养基为 1/2 MS+2.0 mg·L-1 NAA+60 g·L-1土豆泥+0.5 g·L-1活性炭, 培养 10 d,不定芽发生率为 98.33%,培至 40 d,幼苗生根率为 94.67%;试管苗在温度 20℃、光照时间 12 h·d-1、光照强度 2 000 lx 的条件下培养,苗长势好,叶片生理性焦尖发生率仅为 3.33%;以腐殖土作为栽培基质,试管苗的移栽成活率为 97.78%。该研究结果为保护西藏虎头兰野生资源和工厂化育苗提供了科学依据和技术支持。

ymbidium tracyanum is a Orchidaceae species, characterized by a beautiful and showy inflorescence. The species has a great potential for commercial exploitation in the flower industry. Plant tissue culture technology offers an effective tool for C. tracyanum large-scale production to avoid endangering its natural populations, yet there is a few research on the plant tissue culture of C. tracyanum. The current study evaluated the effects of basic medium and hormone combination on the plant regeneration of C. tracyanum. The effects of illumination time and culture temperature on the growth of plantlets were also studied. The results showed that the optimum basic medium for C. tracyanum growth was 1/2 MS. The optimal medium for protocorm induction was 1/2 MS1.0 mgL-1 6-BA0.5 mgL-1 NAA, 95.00% of the seeds developed into the protocorms after cultured for 50 d. The optimal medium for protocorm proliferation was 1/2 MS2.0 mgL-1 NAA, the maximum proliferation multiple of protocorm reached 4.25 after cultured for 30 d. The optimal medium for protocorm differentiation was 1/2 MS2.0 mg L-1 NAA60 g L-1 mashed potato0.5 g L-1acticarbon, the induction rate of indefinite bud was 98.33% after cultured for 10 d, the rooting rate was 94.67% after cultured for 40 d. In the process of plantlets subculture, temperature of 20 , illumination time of 12 hd-1 and illumination intensity of 2 000 lx were suited for the growth of plantlets, the physiological scorch rate of leaf tip was 3.33%. Using humus as cultivation substrate, the transplanting survival rate of plantlets was 97.78%. This study provides an effective way for keeping good varieties of features and rapid propagation of C. tracyanum, at the same time helps to solve some theoretical problems in factory production of C. tracyanum.

袁芳、杨泽东、宋凯杰、王 兵、蔡熙彤、兰小中

10.12074/201808.00148V1

生物工程学植物学

西藏虎头兰,种子,原球茎,组织培养,植株再生

袁芳,杨泽东,宋凯杰,王 兵,蔡熙彤,兰小中.西藏虎头兰高效植株再生体系的研究[EB/OL].(2018-07-18)[2025-08-23].https://chinaxiv.org/abs/201808.00148.点此复制

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