大白菜EMS诱变结合小孢子培养获得突变体

In this study, five different genotypes of Chinese cabbage were used for researching influences on microspore development, production rate of embryo and seedling rate by soaking buds using ethyl methane sulphonate (EMS) with different doses and different duration. The results indicated that microspores wound be lethal when the dose was higher than 0.1%, the production rate of embryo and seedling were high when buds were treated with 0.03 ~ 0.1% of EMS for 5 ~ 10 min and it is suitable for microspore culture after EMS mutagenic buds. The reproductive growth of homozygous plants were investigated, and mutants of flower color, leaf shape, and the resistant mutants of downy mildew were obtained. The results suggested that it is feasible to obtain regeneration plantlets by EMS induced mutation in Chinese cabbage, and provided a basis for creating the new germplasm of Chinese cabbage rapidly.