乳腺炎奶牛整合素a4β7表达的检测

Bovine mastitis is one of the major disease which cause serious economic loss to the dairy farming worldwide. Mammary gland is an important organ to the cow, the lymphocyte can not only against pathogens, but also provide assistance from a passive immunization children through breast milk. Research has shown that the migration of lymphocyte has tissue specificity, which depends on the expression of adhesion molecules on the vascular endothelium and cell surface. To further research the differential expression of lymphocytes homing receptor α4β7 in healthy and mastitis bovine, take blood and milk from healthy and mastitis bovine, respectively, use flow cytometry to detect lymphocytes which positive express α4β7. The results showed that α4β7 were expressed on lymphocytes both in blood and milk from healthy and mastitis bovine, and the positive rate in mastitis bovine was higher than the healthy bovine.