Multinuclear MRI reveals early efficacy of stem cell therapy in stroke
Multinuclear MRI reveals early efficacy of stem cell therapy in stroke
Abstract Compromised adult human mesenchymal stem cells (hMSC) can impair cell therapy efficacy and further reverse ischemic recovery. However, in vitro assays require extended passage to characterize cells, limiting rapid assessment for therapeutic potency. Multinuclear magnetic resonance imaging and spectroscopy (MRI/S) provides near real-time feedback on disease progression and tissue recovery. Applied to ischemic stroke, 23Na MRI evaluates treatment efficacy within 24 h after middle cerebral artery occlusion, showing recovery of sodium homeostasis and lesion reduction in specimens treated with hMSC while 1H MRS identifies reduction in lactate levels. This combined metric was confirmed by evaluating treatment groups receiving healthy or compromised hMSC versus control (sham saline injection) over 21 d. Behavioral tests to assess functional recovery and cell analysis for immunomodulatory and macrophage activity to detect hMSC potency confirm MR findings. Clinically, these MR metrics may prove critical to early evaluations of therapeutic efficacy and overall stroke recovery.
Athey Jacob、Grant Samuel C.、Bagdasarian F. Andrew、Li Yan、Helsper Shannon、Borlongan Cesario V.、Yuan Xuegang
Department of Chemical and Biomedical Engineering, FAMU-FSU College of EngineeringThe National High Magnetic Field Laboratory, Florida State University||Department of Chemical and Biomedical Engineering, FAMU-FSU College of EngineeringThe National High Magnetic Field Laboratory, Florida State University||Department of Chemical and Biomedical Engineering, FAMU-FSU College of EngineeringDepartment of Chemical and Biomedical Engineering, FAMU-FSU College of EngineeringThe National High Magnetic Field Laboratory, Florida State University||Department of Chemical and Biomedical Engineering, FAMU-FSU College of EngineeringCenter of Excellence for Aging & Brain Repair, University of South FloridaThe National High Magnetic Field Laboratory, Florida State University||Department of Chemical and Biomedical Engineering, FAMU-FSU College of Engineering
医学研究方法神经病学、精神病学基础医学
human mesenchymal stem cellsischemic strokemagnetic resonance imagingNMR spectroscopysodiumrat
Athey Jacob,Grant Samuel C.,Bagdasarian F. Andrew,Li Yan,Helsper Shannon,Borlongan Cesario V.,Yuan Xuegang.Multinuclear MRI reveals early efficacy of stem cell therapy in stroke[EB/OL].(2025-03-28)[2025-05-24].https://www.biorxiv.org/content/10.1101/2022.02.08.479630.点此复制
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