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外源亚精胺对盐胁迫下黄瓜幼苗生长和可溶性蛋白表达的影响

Effects of Exogenous Spermdine on the Growth and Soluble Protein Expression in Cucumber Seedlings Under NaCl Stress

中文摘要英文摘要

采用营养液水培的方法,研究了叶面喷施亚精胺(Spd)对黄瓜幼苗生长和可溶性蛋白的影响。结果表明,盐胁迫条件下,外源Spd可使黄瓜幼苗株高、茎粗、叶面积、根长和根表面积显著增加,可溶性蛋白含量明显提高,减弱部分分子量蛋白的表达量。而正常生长条件下,喷施外源Spd对各生长指标无明显影响,但显著提高了根系中可溶性蛋白的含量。盐胁迫下叶面喷施Spd后,随着处理时间的变化,至少有7种分子量分别约为67 kD、61.5 kD、50 kD、47 kD、43 kD、29 kD和16 kD的可溶性蛋白表达发生明显变化,尤其使61.5 kD、47 kD和43 kD蛋白表达量明显减弱,50 kD蛋白甚至消失,由此推断出这4种蛋白与外源Spd缓解黄瓜幼苗盐胁迫伤害关系密切。

Effects of exogenous spermdine (Spd) on growth, the soluble protein content and expression was conducted with salinity- sensitive cucumber (Cucumis sativus L.) cultivar ‘Jinchun No. 2’ by hydroponic culture. Results showed that exogenous Spd significantly increased the growth and the content of soluble protein under 50 mmol•L-1 NaCl stress. Compared with the control, exogenous Spd was not significantly different to growth indexes, but affected significantly soluble protein content in roots. Seven proteins changed obviously at least, such as 67 kD, 61.5 kD, 50 kD, 47 kD, 43 kD, 29 kD and 16 kD protein. In comparion with salt stress, exogenous Spd could decrease significantly the expression of these proteins,61.5kD、47kD and 43 kD,and could not detected 50 kD completely. Exogenous Spd significantly alleviated the injury to seedlings, increased the content of soluble protein and decreased the expression of partial proteins. So it was possible that these proteins have relations with exogenous Spd alleviating the effect of salt stress in cucumber seedlings.

郭世荣、杜长霞、李娟、樊怀福

植物学生物化学农艺学

黄瓜盐胁迫亚精胺可溶性蛋白SDS-PAGE

ucumberNaCl StressSpermdineSoluble ProteinSDS-PAGE

郭世荣,杜长霞,李娟,樊怀福.外源亚精胺对盐胁迫下黄瓜幼苗生长和可溶性蛋白表达的影响[EB/OL].(2009-01-09)[2025-08-02].http://www.paper.edu.cn/releasepaper/content/200901-377.点此复制

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