太湖蓝藻微囊藻毒素的薄层层析研究

Microcystins are a series of hepatotoxins produced by Cyanobacteria. According to various dielectric constants of developing solvents, thin-layer chromatography method was developed for detecting the microcystin MC-LR, MC-RR. The single factor test and response surface analysis were introduced for obtaining the optimum ratio of developing solvents is trichloromethane : methanol : water = 65:25:2. The lowest concentration of MC-LR, MC-RR visually observed using the iodonium development is 1mg•L-1. The lowest concentration of MC-LR, MC-RR distinguished by TLC-scanning determination is 0.001 mg•L-1. The peak values of MC-LR、MC-RR are in a linear relationship to the concentrations of them in the range of 0.05~10 mg•L-1. The accuracy of the detection is above 95％.