Role of the inner membrane cytochrome ImcH in Geobacter extracellular electron transfer and energy conservation
Role of the inner membrane cytochrome ImcH in Geobacter extracellular electron transfer and energy conservation
Abstract Electroactive bacteria combine the oxidation of carbon substrates with an extracellular electron transfer (EET) process that discharges electrons to an electron acceptor outside the cell. This process involves electron transfer through consecutive redox proteins that efficiently connect the inner membrane to the cell exterior. In this study, we isolated and characterized the quinone-interacting membrane cytochrome c ImcH from Geobacter sulfurreducens, which is involved in the EET process to high redox potential acceptors. Our work provides evidence that ImcH is electroneutral, as it transfers electrons and protons to the same side of the membrane, contributing to the maintenance of a proton motive force, and plays a central role in recycling the menaquinone pool. ImportanceGeobacter sulfurreducens is a model electroactive bacterium, widespread in the environment and of significant interest for biotechnological applications. Its ability to form thick and conductive biofilms on top of conducting surfaces makes this microbe very useful in bioelectrochemical systems for the production of energy or added value products. To explore Geobacter spp. as a biocatalyst it is essential to understand its metabolism, particularly the molecular mechanisms for extracellular electron transfer and energy conservation. Our results reveal the importance of ImcH in both processes, identifying this protein as a major player on Geobacter metabolism.
Morgado Leonor、Pimenta Andreia I.、Pereira In¨os A.C.、Edwards Marcus J.、Salgueiro Carlos A.、Clarke Thomas、Duarte Am¨|rico G.、Paquete Catarina M.
Associate Laboratory i4HB - Institute for Health and Bioeconomy, NOVA School of Science and Technology, Universidade NOVA de Lisboa||UCIBIO ¨C Applied Molecular Biosciences Unit, Department of Chemistry / Department of Life Sciences, NOVA School of Science and Technology, Universidade NOVA de LisboaInstituto de Tecnologia Qu¨amica e Biol¨?gica Ant¨?nio Xavier / Universidade Nova de LisboaInstituto de Tecnologia Qu¨amica e Biol¨?gica Ant¨?nio Xavier / Universidade Nova de LisboaSchool of Life Sciences, University of EssexAssociate Laboratory i4HB - Institute for Health and Bioeconomy, NOVA School of Science and Technology, Universidade NOVA de Lisboa||UCIBIO ¨C Applied Molecular Biosciences Unit, Department of Chemistry / Department of Life Sciences, NOVA School of Science and Technology, Universidade NOVA de LisboaCentre for Molecular and Structural Biochemistry, School of Biological Sciences, University of East AngliaInstituto de Tecnologia Qu¨amica e Biol¨?gica Ant¨?nio Xavier / Universidade Nova de LisboaInstituto de Tecnologia Qu¨amica e Biol¨?gica Ant¨?nio Xavier / Universidade Nova de Lisboa
生物化学生物工程学分子生物学
Electroactive bacteriaGeobacterEnergy ConservationExtracellular Electron Transfer
Morgado Leonor,Pimenta Andreia I.,Pereira In¨os A.C.,Edwards Marcus J.,Salgueiro Carlos A.,Clarke Thomas,Duarte Am¨|rico G.,Paquete Catarina M..Role of the inner membrane cytochrome ImcH in Geobacter extracellular electron transfer and energy conservation[EB/OL].(2025-03-28)[2025-08-02].https://www.biorxiv.org/content/10.1101/2023.05.03.539133.点此复制
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