不同氟烷基因型猪肉质与能量代谢的关系

In this study, pigs at 65 kg of body weight differing in halothane genotype (HalNN, homozygous wild type; HalNn, heterozygous; Halnn, homozygous mutant) were employed to investigate the influence of halothane genotype on muscle energy metabolism, in association with meat quality. Results indicated that HalNN pigs exhibited the best pork quality, Halnn showed a slight PSE syndrome, and HalNn were the intermediate. Halnn demonstrated significantly higher glycogen and LD contents in longissimus dorsi compared with HalNN and HalNn (both P < 0.05), while no difference was observed between HalNN and HalNn. Besides, there was significantly lower CK, higher AMPK activities in longissimus dorsi of Halnn than those in HalNN and HalNn (all P < 0.05). Real-time PCR results showed that GLUT4 and PGC-1α transcripts were significantly higher in HalNN compared with Halnn, and no significent difference between HalNn and Halnn was observed. These results indicate that longissimus dorsi muscles from different halothane genotype exhibit distinct metabolic patterns. Also indicate that HalNN pigs have much higher capacity of energy metabolism both in aerobic metabolism and anaerobic glycolysis which may contribute to the better meat quality.