益髓通经方对实验性自身免疫性神经炎大鼠坐骨神经传导速度及超微结构的影响

Objective: To study effect of Yisui Tongjing prescription on the conduction velocity of motor nerve (MNCV) and microstructure of the sciatic nerve in experimental autoimmune neuritis (EAN) rats, the Guillain-Barre Syndrome (GBS) classic animal models. Methods: The Lewis rat models of EAN were made by immunization with P0180-199 and they were randomly divided into 5 groups: the model group, intravenous immune globulin (IVIg) group, high, medium , low dose Yisui Tongjing prescription group. Normal rats were taken as the control group. Each group was treated by different dose of Yisui Tongjing liquid or IVIg injected into the abdominal cavities. MNCV and microstructure of the sciatic nerve were tested with BL－420 biological functional system and electron microscope on the 10th , 18th , 25th , 39th day after immunization.Results: Compared with normal group, the MNCV in model group were significantly decreased on the 10th , 18th , 25th day and little increased on the 39th day. The MNCV in each Yisui Tongjing prescription group was higher than that in model group. On the 39th day, the MNCV showed no significant difference between high dose group and IVIg group, but was higher than that in medium and low dose group（p＜0.01）, and was lower than that in normal group（p＜0.01）. Folds and crinkles of myelin sheath and wide gaps between axon and sheath appeared on the 18th day, demyelination and myelinolysis were obvious on the 25th day and most serious on the 39th day in model group. The above changes were significantly ameliorated in high dose group and in IVIg group after treatment.Conclusion: Yisui Tongjing prescription can increase the MNCV of the sciatic nerves in EAN rats, alleviated the demyelination and promote the nerve repair and regeneration.