“瓜蒌-薤白”药对对大鼠心肌缺血再灌注损伤的保护作用

Objective To investigate the effects of Trichosanthes Kirilowii Maxim and Allium macrostemon Bge (TKAM) on myocardial ischemia reperfusion injury in rats. Methods The ischemic model was made by occluding the anterior descending of the left artery (LAD) in rats. The change of ST segment were observed ; the activity of creatine kinase- MB (CK-MB) in serum were measured; the activity of the lactate dehydrogenase (LDH) and superoxide dismutase (SOD) in serum were measured; and the levels of maleic dialdehyde (MDA) in serum were measured. And by tetrazolium chloride (TTC) Staining , the areas of myocardial infraction were observed. The cardiac muscle tissue was stained by HE to observe its necrosis area and pathological changes. Results On the myocardial infarction model by occluding the anterior descending of the LAD in rats, TKAM obviously protected the myocardium cells , reduced the height of ST segment and the areas of myocardial infraction , and decreased the activity of CK-MB, LDH in serum ,and decreased the level of MDA in serum, and enhanced the activity of SOD in serum. Conclusions After Trichosanthes Kirilowii Maxim compatibility with Allium macrostemon Bge ,the whole protection against myocardial ischemia-reperfusion injury has enhanced.