GhIDD57调控棉花黄萎病抗性机理研究

Verticillium wilt seriously harms cotton production, so it is of great significance to analyze the mechanism of resistance to verticillium wilt in cotton. The INDETERMINATEDOMAIN (IDD) family is a family of plant-specific transcription factors. Our previous study found that GhIDD57 can negatively regulate cotton verticillium wilt resistance, but the specific regulatory mechanism remains unclear. In this study, virus-induced gene silencing (VIGS) technique was used to silence GhIDD57 in cotton, and the silenced plant leaves were sequenced, and the GhIDD57 binding promoter motif was analyzed with bioinformatics, and GhMYB3 was screened as its downstream target gene. The expression of GhMYB3 was significantly increased in GhIDD57 silenced plants and significantly decreased in cotton protoplasts with overexpression of GhIDD57 by RT-qPCR. The results of yeast single hybridization analysis showed that GhIDD57 can directly bind to GhMYB3 promoter motif and inhibit GhMYB3 expression, indicating that GhMYB3 is downstream and negatively regulated by GhIDD57.