三种乳化剂对厚朴酚抑制变形链球菌生长及产酸作用影响的体外研究

Objective：To study the influence of emulsifiers on inhibitory effect of the magnolol on streptococcus mutans and its mechanisms by studying the inhibitory effects of magnolol on the growth and acid production of streptococcus mutans under solubilization of three emulsifiers - polyethylene glycol 400, Tween20, Tween80. It can be a reference to selecting emulsifier when the anti-caries drug prescription can be made in Magnolia. Method: Magnolol was dissolved in 40% solution of three kinds of emulsifier, and proportionally diluted to the preset concentration with solution of emulsifier. Then the effects of the agent on the growth of the bacteria were assessed by slip diffusion method. 2. Magnolol was dissolved in 40% solution of three kinds of emulsifier, and proportionally diluted to the preset concentration with MS liquid media. The same amount of bacteria was added into the MS liquid medium and was cultured to study the inhibition effect of production acid of streptococcus mutans. Result: 1. At high concentration, magnolol under solubilization of polyethylene glycol 400 had better inhibitory effect on the growth of streptococcus mutans. At low concentration, magnolol under solubilization of Tween80 had better inhibitory effect. 2. Magnolol under solubilization of polyethylene glycol 400 had best inhibitory effect on acid production of streptococcus mutans, and magnolol under solubilization of Tween80 had worst inhibitory effect. Conclusion: Magnolol under solubilization of different emulsifiers has different inhibitory effect on the growth and acid production of streptococcus mutans. Increasing the lipophilic chain in emulsifiers increased the inhibitory effect on the diffusion of magnolol.