新一代测序技术及其在肿瘤研究中的应用

he next-generation sequencing (Next Generation Sequencing, NGS), also known as high-throughput sequencing technology, is a landmark of revolution in the history of DNA sequencing. Next-generation sequencing technology have adopted a new sequencing strategy-cyclic-array sequencing, divided into pyrosequencing (Roche/454), Solexa sequencing technology (Illumina) the polony sequencing technology (ABI/ SOLiD), Polonator sequencing technology and HeliScope single-molecule sequencing technology. Because of its high-throughput, low cost and short time-consuming it has replaced traditional Sanger sequencing became the important research tools in post-genomic era. Researchers could do more comprehensive and in-depth analysis with genome, transcriptome and proteome at cheaper prices. Next-generation sequencing has broad application prospects in the field of cancer research, in addition to quickly realize the tumor genome sequencing, but also provide us a fully digital rapid powerful tool for tumor transcriptome, epigenetic and DNA-protein interaction analysis. It was expecting to bring about revolutionary change in the life sciences, medicine science and other fields.