Resovist磁化标记大鼠骨髓间充质干细胞的有效性和安全性研究

Objective：To explore the effectiveness and safty of labeling rat bone marrow mesenchymal stem cells(MSCs) with Resovist, a kind of superparamagnetic iron oxides(SPIO)．Methods：MSCs isolated from Sprague-Dawley rat were incubated with Resovist nanoparticles at the concentration of 50 μg/ml for 24 hours．The labeling effectiveness of Resovist particles in MSCs was assessed by Prussian BIue staining, electron microscopy and atomic absorption spectrometry．The Cell toxity of Resovist particles was observed by the proliferation assays and cellular viability with the help of a CASY?2 Analyser．The cellular effect of a 0.6T magnetic fields on MSCs were also assessed. Results：The Prussian blue staining of the MSCs showed intracytoplasmic iron inclusions as dense blue-stained vesicles, and the labeling efficiency was approximately 100%. Transmission electron microscopy of labeled cells indicated the presence of anionic magnetic nanoparticles exclusively in polydisperse vesicles in the cytoplasm, and no obvious change in the ultramicrostructure was observed. The average cellular iron content was 21.77 ± 3.62 pg per cell after 24 hours of incubation. The proliferation rate and viability rate showed no differences between unlabeled MSCs and labeled MSCs with and without exposure to magnetic fields. Conclusion：Resovist could effectively and safely label rat bone marrow stem cells, which may be useful in cellular magnetic targeting strategies.