二维硼纳米片的类过氧化物酶性质及用于过氧化氢的比色测定

wo-dimensional boron nanosheets (2DBS) with excellent intrinsic peroxidase-like activity were prepared in this research. Based on the nanozyme that can catalyze hydrogen peroxide oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) to generate a colored solution, a rapid colorimetric detection method for hydrogen peroxide in milk was established. The results of the Michaelis-Menten model showed that the affinity of 2DBS for hydrogen peroxide was much higher than that of cobalt oxide, ferroferric oxide nanoparticles, and horseradish peroxidase (HRP). In addition, the catalytic mechanism was investigated. Under the optimal conditions, the colorimetric method showed good linearity in the range of 0.01~0.2 μM and the detection limit of hydrogen peroxide was 1.26 nM. The recovery rate of hydrogen peroxide in milk samples was in the range of 89.7%~105.37% and the relative standard deviation was <2.1%. The coexisting substances in the milk sample hardly interfere with the colorimetric method, and the detection results can fully meet the detection requirements of actual samples.