利用单克隆抗体研究RG-I型人参果胶结构特征

In this paper, we have employed monoclonal antibodies in combination with acid hydrolysis and NMR spectroscopy to assess the fine structures of two ginseng rhamnogalactouronan-I (RG-I) domains. Our results indicate that both RG-I species have typical RG-I backbone structures with [→4)-α-GalA-(1→2)-α-Rha-(1→] repeat units, respectively, in addition to neutral and acidic side chains. The neutral side chains in both species are similar with arabinan, galactan, and AG-I/II structures linked to the RG-I backbone via C-4 of Rha residues. However, the lengths of their acidic GalA moieties, linked via side chain Ara and Gal sugars, are different. Our studies provide significant insight into the chemical structure of RG-I type pectins, information that may impact on their function in the development of pectin-based therapeutics.