广西三种真红树植物可培养细菌多样性及其生物活性初筛

his study aims to explore potential new bacterial species and bioactive substances in mangrove plants, which can enrich the diversity of mangrove microorganisms and provide strain resources for the development of new active products. 22 samples were collected from three true mangrove plants of Kandelia candel, Bruguiera gymnorrhiza, Rhizophora stylosa, and their habitats, in Guangxi coastal area. Then 22 samples were divided according to different parts such as roots, stems, leaves, flowers, fruits and muds. Eight different culture mediums were used to isolate culturable bacteria, and 16S rRNA gene sequences were used to identify bacteria and analyze diversity. For cultured bacteria, the antibacterial and enzyme activities of fermented crude extracts were screened with Kirby-Bauer method and spot planting method, respectively. The results were as follows:1Based on sequencing results of 16S rRNA gene, a total of 35 culturable bacteria were isolated, belonging to 28 genera and 23 families. Bacillus accounted for 14.3% of the total bacteria, which was the dominant bacteria. Meanwhile, 11 potential new bacterial species were found. 2Four strains of bacteria showed antibacterial activities, 16 strains had enzyme activities, and Bacillus was the dominant strain in enzyme activities. The above results showed that Guangxi true mangrove plants were rich in bacterial diversity, and some bacteria showed antibacterial activities and enzyme activities. The new bacterial species and active strain had certain development potential in the new antibiotics and enzymes application.