TGF-β诱导miR-181a表达以及与肝癌转移关系的研究
The study on expression of miR-181a inducted by TGF-β and its function on HCC metastasis
摘要
目的：为了探索miR-181a是否受TGF-β诱导及其对肝癌转移的作用。
方法：通过real-time PCR检测TGF-β处理后的肝癌细胞中miR-181a的表达情况，进一步探讨了其作用机制；同时还检测了肝癌标本中miR-181a的表达并分析了其临床意义；此外通过transwell实验方法检测了miR-181a对肝癌细胞侵袭能力的影响。结果：Real-time PCR结果显示TGF-β直接诱导miR-181a的表达并依赖于Smad2途径；miR-181a在肝癌组织中的表达高于癌旁和正常肝组织，且与转移密切相关；侵袭实验结果进一步证实miR-181a体外可促进肝癌细胞侵袭。结论：本文揭示了miR-181a的表达受到TGF-β的诱导，且与肝癌转移相关；miR-181a体外可增强肝癌细胞的侵袭能力；以上发现为肝癌的治疗提供了一个新的潜在靶点。Abstract
Objective: To reveal the response of miR-181a expression to TGF-β presence and the function of miR-181a on hepatocellular carcinoma. Method: The relative expression of miR-181a in HCC cells treated with TGF-β was tested by real-time PCR. Moreover, the expression level of miR-181a in HCC samples and the clinical significance were analyzed. Furthermore, the effect of miR-181a on HCC cell invasion was detected through transwell assays coated with matrigel. Result: TGF-β induces miR-181a expression which was dependent on the Smad2 pathway; miR-181a was upregulated in HCC tissues compared to noncancerous tissues and normal liver tissues, and its expression was correlated with metastasis of HCC. Additionally, miR-181a could promote HCC cell invasion in vitro.
Conclusion: In this paper, it was discovered that TGF-β induced miR-181a expression which was related to HCC metastasis and could enhance the HCC cell invasion. Thease findings provide a potential target for HCC therapy. 关键词
miR-181a/TGF-β/转移/肝细胞癌Key words
miR-181a/TGF-β/metastasis/hepatocellular carcinoma引用本文复制引用
田琦,何祥火,黄胜林,顾健人,丁洁.TGF-β诱导miR-181a表达以及与肝癌转移关系的研究[EB/OL].(2012-05-07)[2025-12-13].http://www.paper.edu.cn/releasepaper/content/201205-90.学科分类
肿瘤学/基础医学/分子生物学
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