raurine抑制破骨细胞分化的同时不影响成骨细胞OPG与RANKL的表达

Objective: Taurine is widely distributed in mammalian plasma andtissues, it is the most important free beta-amino acids in mammals. Ourprevious studies have found that Taurine could inhibit osteoclastogenesis,but the exact mechanism is still unknown. In this study, osteoblasts andbone marrow cells were co-cultured, in order to investigate whetherTaurine inhibits osteoclastogenesis by affecting the expression of OPGand RANKL in osteoblasts. 　　 Methods: Skull of 24 hour newborn mouse and long bone of6-8week aged mouse were isolated for osteoblast and bone marrow cell,respectively.The osteoblast and bone marrow cells were then co-culturedwith different concentrations of Taurine for 6 days TRACP staining wereperformed for validation and counting of the osteoclasts. The expressionof OPG and RANKL in osteoblast were then validated by real-time PCR. Results: Taurine dose-relatively inhibited osteoclastogenesis inco-culture of osteoblasts and bone marrow cells, but Taurine showed noinfluential effect to the expression of OPG and RANKL in osteoblasts.Conclusion: Taurine inhibits osteoclastogenesis in a dose-dependentmanner in co-culture of osteoblast and bone marrow cell. This inhibitoryeffect had no relationship with respect of OPG and RANKL in osteoblast.KEY WORDS Taurine, osteoclastogenesis, OPG, RANKL.