芍药甘草汤大鼠血中移行组分的研究

OBJECTIVE To study the adscription of plasma effective constituents of rat after oral administration of Shaoyao-gancao decoction ( SGD ) by HPLC-DAD-MS/MS, and confirm the effective constitutes in plasma. METHODS kormasilC18 (4.6250 mm, with protection column) was chromatographic column; acetonitrile and 0.5 ％ acetic acid/water was mobile phase for gradient elution, flow rate was 0.8 mL•min-1, column temperature: 25 ℃，methanol was used to remove the protein, A LCQdeca mass spectrometer system equipped with an electrospray ionization iontrap source was used as the detector and operated in the positive ion mode. Ions was scanned from the m/z 100 to m/z 1000, and characteristic ion were secondary schizolysised to obtain data of second order MS. RESULTS The main constitutes of SGD were peoniflorin, Albiflorin R1, Liquiritin and glycyrrhizic acid and so on, but no glucosides was found in the plasma samples of rats administered with SGD, glucuronide conjunctions of liquiritigenin and isoliquiritigenin were detected in plasma sample. CONCLUSION liquid chromatography-mass spectrometry technology provides a convenient and high speed method to analyze the effective constitutes of plasma of rats administered with SGD, therefore, it has a good value in studies on the metabolism of traditional Chinese medicine ( TCM ).