肝细胞生长因子（HGF）对TGF-b1诱导的肌腱成纤维细胞的增殖、凋亡的作用

Background Due to the complex nature of the tendon architecture, the regeneration of these tissues results in the formation of scars. As a direct result of scar formation, the ability of the tendon tissues to function is impaired and often results in further damage that has been afflicted to the tendon architecture. The growth and proliferation of tendon fibroblasts involve a complex network of signal molecules. To understand and aid in the proper repair of this complex tissue network, a more in-depth understanding is required in the events that induce the growth of tendon cells. Several studies have shown the apoptotic mechanisms induced by the mitogen, hepatocyte growth factor, in multiple biological and pathological systems. In our recent research, we have described a mechanism where hepatocyte growth factor (HGF) is able to inhibit the proliferative effects of transforming growth factor-β1 (TGF-β1) and induce apoptosis in rat tendon fibroblasts. Methods Tendon fibroblasts of rats were harvested to primary culture in vitro, which were induced fibrosis response by the addition of transforming growth factor β1 (TGF-β1) (5 ng/ml). Under conditions with or without the intervention of hepatocyte growth factor (HGF), cell proliferation, RNA expression, total protein expression, and tendon fibroblast apoptosis were measured. Results HGF is able to inhibit the proliferative effects of TGF-β1 and induce apoptosis in rat tendon fibroblasts. Conclusions HGF suppress TGF-β1 induced fibroblasts proliferation by cell apoptosis.