电磁脉冲对大鼠大脑皮层蛋白激酶C含量的影响
Effect of electromagnetic pulse on the expression of protein kinase C in the rat cerebral cortex
目的 探讨电磁脉冲（electromagnetic pulse, EMP）对成年雄性SD大鼠大脑皮层中蛋白激酶C（protein kinase C, PKC）含量的影响。方法 将成年雄性SD大鼠随机分为假辐照组和辐照组，辐照组又按辐照后取材时间不同分为0.5 h, 1 h, 3 h, 6 h和12 h。采用酶联免疫吸附实验（enzyme-linked immunosorbent assay, ELISA）法分析大脑皮层中总PKC含量；选用Western Blot法分析PKC（PKC-α, PKC-βⅠ, PKC-βⅡ）, PKC-α和PKC-βⅡ蛋白含量。结果 大脑皮层中总PKC含量在EMP辐照后0.5 h增加且达到峰值（P < 0.05），之后逐渐恢复，至12 h达到假辐照组水平（P > 0.05）；Western Blot结果显示 PKC及PKC-βⅡ在EMP辐照后0.5 h和1 h表达量增加（P < 0.05），之后恢复至假辐照组水平，PKC-α在辐照各组与假辐照相比均不具有统计学差异（P > 0.05）。结论 EMP辐照可以引起大鼠大脑皮层中PKC含量可逆性增加，间接反映其活性增加。
IObjective To study the effect of electromagnetic pulse (EMP) on the expression of protein kinase C (PKC) in the cerebral cortex of adult male SD rats. Methods Male SD rats were sham or whole-body exposed to EMP at 200 kV/m for 200 pulses (1 Hz). At 0.5 h, 1 h, 3 h, 6 h, 12 h after EMP exposure, total PKC content in the cerebral cortex was estimated by enzyme-linked immunosorbent assay (ELISA), and the expressions of PKC (PKC-α, PKC-βⅠ, PKC-βⅡ), PKC-α and PKC- βⅡ were determined by Western Blot method. Results Total PKC content was increased at 0.5 h, 1 h, 3 h and 6 h after EMP irradiation compared with sham group (P < 0.05), and the peak was at 0.5 h group. It recovered to the level of sham group at 12 h (P > 0.05). Western Blot showed that the expressions of PKC and PKC-βⅡ were increased at 0.5 h and 1 h after EMP exposure compared with sham group (P < 0.05). PKC-α was not significant changed (P> 0.05). Conclusion EMP could cause the PKC content increased reversible in rat cerebral cortex.
陈晨、丁桂荣、邱联波、丁军伟
基础医学生物科学现状、生物科学发展生物科学研究方法、生物科学研究技术
电磁脉冲蛋白激酶C酶联免疫吸附实验Western Blot
electromagnetic pulseprotein kinase Cenzyme-linked immunosorbent assayWestern Blot
陈晨,丁桂荣,邱联波,丁军伟.电磁脉冲对大鼠大脑皮层蛋白激酶C含量的影响[EB/OL].(2010-09-19)[2025-08-11].http://www.paper.edu.cn/releasepaper/content/201009-412.点此复制
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