Single cell transfection of human induced pluripotent stem cells using a droplet-based microfluidic system
Single cell transfection of human induced pluripotent stem cells using a droplet-based microfluidic system
ABSTRACT Microfluidic tools have recently made possible many advances in biological and biomedical research. Research fields such as Physics, Engineering, Chemistry and Biology have combined to produce innovation in Microfluidics which has positively impacted on areas as diverse as nucleotide sequence, functional genomics, single-cell studies, single molecules assays, and biomedical diagnostics. Among these areas regenerative medicine and stem cells have benefited from Microfluidics due to these tools have had a profound impact on their applications. In the study, we present a high-performance droplet-based system for transfecting individual human-induced pluripotent stem cells. We show that this system has great efficiency in single cells and captured droplets, similar to other microfluidic methods and lower cost. We demonstrate that this microfluidic approach can be associated with the PiggyBac transposase-based system to increase its transfection efficiency. Our results provide a starting point for subsequent applications in more complex transfection systems, single-cell differentiation interactions, cell subpopulations, cell therapy, among other potential applications.
P¨|rez C.、Bhansali S.、Bourguignon N.、Rosero G.、Lombardi A.、Luzzani C.、P¨|rez M.S.、La Greca A.、Waisman A.、Miriuka S.、Lerner B.、Sanluis-Verdes A.
Universidad Tecnol¨?gica Nacional (UTN), Facultad Regional HaedoDepartment of Electrical and Computer Engineering, Florida International UniversityUniversidad Tecnol¨?gica Nacional (UTN), Facultad Regional Haedo||Department of Electrical and Computer Engineering, Florida International UniversityUniversidad Tecnol¨?gica Nacional (UTN), Facultad Regional HaedoConsejo Nacional de Investigaciones Cient¨aficas y T¨|cnicas - Fundaci¨?n para la Lucha contra las Enfermedades Neurol¨?gicas de la Infancia, (LIAN-CONICET-FLENI), FLENI Sede EscobarConsejo Nacional de Investigaciones Cient¨aficas y T¨|cnicas - Fundaci¨?n para la Lucha contra las Enfermedades Neurol¨?gicas de la Infancia, (LIAN-CONICET-FLENI), FLENI Sede EscobarUniversidad de Buenos Aires (UBA), Instituto de Ingenier¨aa Biom¨|dica||Department of Electrical and Computer Engineering, Florida International UniversityConsejo Nacional de Investigaciones Cient¨aficas y T¨|cnicas - Fundaci¨?n para la Lucha contra las Enfermedades Neurol¨?gicas de la Infancia, (LIAN-CONICET-FLENI), FLENI Sede EscobarConsejo Nacional de Investigaciones Cient¨aficas y T¨|cnicas - Fundaci¨?n para la Lucha contra las Enfermedades Neurol¨?gicas de la Infancia, (LIAN-CONICET-FLENI), FLENI Sede EscobarConsejo Nacional de Investigaciones Cient¨aficas y T¨|cnicas - Fundaci¨?n para la Lucha contra las Enfermedades Neurol¨?gicas de la Infancia, (LIAN-CONICET-FLENI), FLENI Sede EscobarUniversidad Tecnol¨?gica Nacional (UTN), Facultad Regional Haedo||Department of Electrical and Computer Engineering, Florida International UniversityUniversidad Tecnol¨?gica Nacional (UTN), Facultad Regional Haedo
生物科学研究方法、生物科学研究技术生物工程学细胞生物学
MicrofluidicSingle CellDropletsTransfectionhiPSCs
P¨|rez C.,Bhansali S.,Bourguignon N.,Rosero G.,Lombardi A.,Luzzani C.,P¨|rez M.S.,La Greca A.,Waisman A.,Miriuka S.,Lerner B.,Sanluis-Verdes A..Single cell transfection of human induced pluripotent stem cells using a droplet-based microfluidic system[EB/OL].(2025-03-28)[2025-08-02].https://www.biorxiv.org/content/10.1101/2020.06.09.143214.点此复制
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