三碘甲腺原氨酸对于高糖作用后RIN-m5F细胞的保护作用
he cytoprotective effect of 3,5,3'-Triiodothyronine (T3) on RIN-m5F cultured with high glucose
目的：探究三碘甲腺原氨酸（T3）对高糖毒性引起的RIN-m5F细胞氧化损伤的保护作用。 方法：传代培养RIN-m5F细胞，分为正常对照组、高糖组（44mM葡萄糖）、α-硫辛酸组（200μM），T3作用组（浓度分别为10-5M，10-7M，10-9M），培养72h后，进行以下测定：DCFH-DA探针检测细胞内ROS水平；MTT法测定细胞存活率；丙二醛（MDA）、试剂盒测定细胞内丙二醛（MDA）含量、超氧化物歧化酶（SOD）活性和总抗氧化能力（T-AOC）；ELISA试剂盒检测葡萄糖刺激胰岛素的分泌水平（GSIS）；RT-PCR检测 MafA、Glut2、GCK mRNA表达量的改变。结果：高糖组与Control组比较，细胞存活率和SOD、T-AOC显著降低，ROS水平和MDA含量显著升高，葡萄糖刺激的胰岛素分泌量减少，MafA、Glut2、GK的mRNA表达量降低， LA的添加可以明显抑制高糖引起的上述变化，而T3除了不能改变高糖诱导细胞GCK基因的表达外均可以抑制其他的变化，且抑制作用存在剂量效应关系。结论：T3对高糖导致的胰岛细胞的氧化损伤有一定的保护作用，且存在浓度依赖性。
Objective To investigate the effect of 3, 5, 3'-Triiodothyronine (T3) on RIN-m5F cultured with high glucose. Methods RIN-m5F were cultured in RPMI-1640, and then divided into normal control group, high glucose group (glucose 44mM), α-lipoic acid group (200μM LA+ high glucose) , T3 groups (10-5M，10-7M，10-9M + high glucose) respectively. The cell viability, the level of intracellular ROS, MDA, SOD, T-AOC, insulin secretion and the mRNA expression level of MafA, Glut2, GCK were measured when cultured for 72h. Results Compare to control group, cell viability, SOD and T-AOC activity were significantly decreased , ROS generation and MDA content were significantly increased ,insulin secretion was significantly lower and the MafA , Glut2 , GK mRNA level were decreased in high glucose group.These changes were inhibited by LA and T3 with a dose-dependent manner, besides T3's effect on the mRNA level of GCK. Conclusion T3 protected RIN-m5F cell against injury induced by high glucose through inhibiting oxidation in dose-dependent manner.
乐国伟、高翠翠、唐雪、蔺忆、施用晖
基础医学生理学生物化学
3氧化应激高糖胰岛素RIN-m5FMafAGlut2GCK
3Oxidative stressHigh glucoseInsulinRIN-m5FMafAGlut2GCK
乐国伟,高翠翠,唐雪,蔺忆,施用晖.三碘甲腺原氨酸对于高糖作用后RIN-m5F细胞的保护作用[EB/OL].(2014-03-25)[2025-08-16].http://www.paper.edu.cn/releasepaper/content/201403-722.点此复制
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