阴道非白念珠菌的PCR-RFLP鉴定
Identification of non-Candida albicans from Patients with Vovovaginal Candidasis by PCR-RFLP
目的 对分离自外阴阴道念珠菌病（VVC）患者阴道的常见致病性非白念珠菌进行PCR–RFLP鉴定。 方法 首先采用芽管试验、厚壁孢子试验、CHROMagar显色培养基及API试纸条将分离自VVC患者阴道内的念珠菌菌株鉴定到种，然后采用真菌通用引物将4种常见非白念珠菌（包括光滑念珠菌、近平滑念珠菌、克柔念珠菌和热带念珠菌）进行PCR扩增，并选用MspⅠ、HaeⅢ两种内切酶对扩增产物进行酶切分析。 结果 在4种非白念珠菌中光滑念珠菌15株（7.5％），近平滑念珠菌7株(3.5%)，克柔念珠菌5株(2.5%)，热带念珠菌2株(1.0%)；PCR扩增后均产生稳定、清晰的条带，扩增产物经MspⅠ、HaeⅢ酶切后分别产生4种和2种特异性带型。 结论 外阴阴道念珠菌病非白念珠菌中以光滑念珠菌为主；PCR–RFLP可以快速、特异的鉴定非白念珠菌。
bstract： Objective To identify common pathogenic non-Candida albicans clinical isolated from patients with vulvovaginal candidasis by PCR-RFLP analysis method. Method Germ tube test,chlamydospore test,CHROMagar Candida and API20 kit system were applied to separate non-Candida albicans strains from Candida albicans.Then PCR was used to amplify the internal transcribed spacer region of ribosomal DNA from 4 species of common pathogenic non-Candida albicans(Candida glabrata,Candida parapsilosis,Candida krusei and Candida tropicalis),and the products were digested with the two restriction endonucleases(MspⅠ、HaeⅢ)respectively. Results The 4 isolates consisted of 170 strains of Candida albicans(85.0%), 15 strains of Candida glabrata(7.5%),7 strains of Candida parapsilosis(3.5%),5 strains of Candida krusei(2.5%),2 strains of Candida tropicalis(1.0%)Distinct bands of products with different molecular weights were obtained after amplification from 4 non-Candida albicans ,and the products were digested with MspⅠand HaeⅢ,producing 4 and 2 unique band patterns,respectively. Conclusion C.glabrata is the main pathogenic yeasts of non- Candida albicans in the vulvovaginal candidiasis.PCR-RFLP is rapid and specific for the identification of non- Candida albicans.
武燕、白瑛、魏玉平、牛桃香、武三卯、骆志成
妇产科学医学研究方法基础医学
非白念珠菌聚合酶链反应多态性，限制性片段长度
non-Candida albicansPolymerase chain reactionPolymorphism，restriction fragment length
武燕,白瑛,魏玉平,牛桃香,武三卯,骆志成.阴道非白念珠菌的PCR-RFLP鉴定[EB/OL].(2008-04-15)[2025-08-11].http://www.paper.edu.cn/releasepaper/content/200804-513.点此复制
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