应用EST-PCR技术鉴定裸燕麦远缘杂交新品系200242

o breed new germplasm resources, made intersoecific distant hybridyzation between wild oat (Avena fatua L., as male,P1) and naked oat (P2) ,then the f1 was used as male crossed by another naked oat variety(P3),3 f2(f21,f22,f23) were selected as emerging varieties.In order to obtain the evidence of hybrid, put the EST-PCR molecular markers in use. The main results show that : (1) Obvious paternal genetic characteristics: There are three primers (AF7, AF8, AF10) simultaneously detected five P1 features bands in f1 and f2 and AF8 detected two only in f2 but no in f1 which affirmed f1 and f2 are descendants of wild oats , primers AF7, AF8, AF10 can be used as specific primers to identify common wild oat offspring. ( 2 ) 12 effective primers amplified 177 bands , in which 135 were polymorphic , accounting for 76.3%. Similarity analysis : P1 and P2: 0.4565, P1 and P3: 0.4347, P2 and P3: 0.6739, f1 and P1: 0.5000, f1 and P2: 0.6522, declared that wild oat and cultivated oats were distantly related , f1 was the distant hybridization offspring of P1&P2. The same as f2 and f1, P3. (3) f21 and f1, P1 were 0.8043, 0.4782;f22 and f1, P1 were 0.5435,0.6087,;f23 and f1, P1 were 0.7174,0.5217 : indicating that the offspring had wide genetic recombination and separation. The import of wild oat genes was multicharacter breeding approaches, distant hybridization technique proved successful.