芦荟大黄素抑制LPS诱导RAW264.7细胞释放炎症因子作用机制研究
he mechanism of Aloe-emodin inhibited the production of pro-inflammatory cytokines in LPS-induced RAW 264.7 cells
目的：以LPS诱导的RAW264.7细胞炎症模型，探讨芦荟大黄素的抗炎作用机制。方法：以5μΜ、10μΜ、20μΜ三个浓度的芦荟大黄素预处理RAW264.7细胞1h后，给予1μg/mL的LPS诱导24h。以ELISA法检测IL-1β、IL-6的生成量，以qRT-PCR方法检测IL-1β、IL-6 mRNA表达。结果：芦荟大黄素中、高剂量组可显著抑制IL-1β、IL-6的生成量（P<0.05，P<0.001），IL-1β、IL-6 mRNA的表达（P<0.05，P<0.001），且均随浓度增加抑制作用增强。结论：芦荟大黄素具有较好的抗炎活性，可抑制炎症细胞因子IL-1β、IL-6的释放，该活性通过抑制它们的转录表达发挥的作用。
Objective: To explore the anti-inflammatory mechanism of aloe-emodin in LPS-induced RAW264.7 cells. Methods: RAW264.7 cells treated with 1μg/mL of LPS for 24h after pretreated with aloe-emodin for 1h which is three concentrations (5μΜ, 10μΜ, 20μΜ). The amount of IL-1βand IL-6 was detected by ELISA assay. The expression of IL-1βand IL-6 mRNA was detected by qRT-PCR. Results: Aloe-emodin significantly inhibited the release of IL-1βand IL-6 (P <0.05, P<0.001), the expression of IL-1βand IL-6 mRNA (P<0.05, P<0.001) in a dose-dependent manner. Conclusion: Aloe-emodin has excellent anti-inflammatory activity, and the mechanism depends on inhibit the transcriptional activity of pro-inflammatory cytokines.
陈小睿、张海、胡泊洋、王平
基础医学生物科学研究方法、生物科学研究技术生物化学
芦荟大黄素RAW264.7巨噬细胞白介素1β白介素6
loe-emodinRAW264.7 macrophageIL-1βIL-6
陈小睿,张海,胡泊洋,王平.芦荟大黄素抑制LPS诱导RAW264.7细胞释放炎症因子作用机制研究[EB/OL].(2014-01-24)[2025-06-06].http://www.paper.edu.cn/releasepaper/content/201401-1119.点此复制
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