609诱导小鼠骨髓间充质干细胞向神经元样细胞分化

In this paper, we treated mouse Bone Mesenchymal Stem Cells (mBMSCs) with D609 to detect whether D609 can induce mBMSC differentiation to neuron-like cell. BMSCs were isolated and cultured as described [1]; the cell morphological changes were observed under a phase contrast microscope; the expressions of neural special markers, NSE and NF, were analyzed by immunofluorescence assay; the changes of ROS levels were analyzed by the fluorescence probe, DCHF. We found that D609 could induce neuronal differentiation of mBMSCs, and the differentiation cells expressed neuronal special markers, NSE and NF. ROS level was increased during this process, indicating ROS may be involved in this differentiation process. FGF-2 could increase the viabilities of differentiation cells by reducing ROS level.