苏云金芽胞杆菌dlt操纵子功能研究

lt operon are widespread in Bacillus, mainly composed of four structural genes dltA, dltB, dltC and dltD ，and its encoded protein can modify cell wall of the main components of the teichoic acid alkylation, formed on the surface of the bacterial cell wall, make a net positive charge, so that the bacillus of cationic antimicrobial peptide resistance increased. This study attempts to construct Bacillus thuringiensis dlt operon mutant, and the characters of the control cell surface charge were determined. By homologous recombination technology knockout of Bacillus thuringiensis acrystalliferous mutant strain BMB171 the dlt operon, compare the difference of the parent strain and the mutant strain growth, cell wall surface charge differences. This study successfully constructed the BMB171 dlt operon mutant, and the traits were determined. To verify the dlt operon also can make the surface of Bacillus thuringiensis net positive charge is increased, which laid a good foundation for subsequent further research of Bacillus thuringiensis dlt operon of biological function, in order to improve the Bacillus thuringiensis insecticidal toxicity to find new ideas.