基于NR2B基因探讨疏肝与补肾法对吗啡精神依赖的调节作用及机制

Objective: To analyze the effect and mechanism of liver-smoothing therapy and kidney-nourishing therapy to morphine-induced psychological dependence. Methods: Morphine was injected to set up the CPP model in rats, Obersiver 5.0 Behavioral Software was used to analyze the time of staying in morphine-paired compartment, and real time PCR was used to detect the mRNA of NR2B. Result: The model group, compared to control group, the time of staying in morphine-paired compartment was significantly up regulated (P<0.01), and the mRNA of NR2B in nucleus accumbens(NAc) and prefrontal cortex(PFC) was up regulated as well (P<0.01 and P<0.05). Compared to model group, in liver-smoothing therapy group, both the time of staying in morphine-paired compartment and mRNA of NR2B were significantly reduced (P<0.05), but in kidney-nourishing therapy group, only the time of staying in morphine-paired compartment was significantly reduced (P<0.01). Conclusion: ①The morphine-induced CPP can be reduced by both liver-smoothing therapy and kidney-nourishing therapy. ②The regulating mechanism of liver-smoothing therapy to morphine-induced CPP may be related to the level of mRNA of NR2B in NAc and PFC. ③The regulating mechanism of kidney-nourishing therapy to morphine-induced CPP should not be related to the level of mRNA of NR2B in NAc and PFC.