葡萄糖对鹅原代肝细胞增殖的影响
Effect of glucose on cell proliferation of goose primary hepatocytes
［目的］为了探讨葡萄糖对鹅肝细胞增殖能力的影响，［方法］本实验通过用葡萄糖培养鹅原代肝细胞，Brdu-ELISA法检测肝细胞DNA含量，Brdu免疫荧光染色检测Brdu阳性细胞率，荧光定量PCR检测细胞增殖相关因子（Cyclin D1、Cyclin D2和Cyclin D3）的mRNA表达量；ELISA法检测Cyclin D1蛋白浓度。［结果］结果表明：与对照组比较， 20 mmol/L和35mmol/L葡萄糖显著增加Brdu阳性细胞率； 20 mmol/L和35 mmol/L可以显著促进原代肝细胞的DNA含量； 5 mmol/L和20 mmol/L葡萄糖对Cyclin D1的蛋白浓度影响不显著，而35 mmol/L葡萄糖对Cyclin D1的蛋白浓度有显著的促进作用； Cyclin D1、Cyclin D2和Cyclin D3的mRNA表达量随着葡萄糖浓度的升高而升高，其中，35 mmol/L葡萄糖的促进作用显著大于其他浓度。［结论］因此，葡萄糖能够促进鹅原代肝细胞的增殖。
Purpose] In order to detect the effect of glucose on the proliferation capability of the goose hepatocytes, [Meathod] goose primary hepatocytes were isolated from Tianfu Meat goose and treated with glucose. The DNA synthesis, the rate of Brdu positive cells, the relative mRNA level and protein content of genes involved in cell proliferation were measured. [Result]The result indicated, compared with control group, the Brdu immunofluorescence staining showed that 20 mmol/L and 35 mmol/L glucose significantly increased the rate of Brdu positive cells; The Brdu-ELISA study showed, 20 mmol/L and 35 mmol/L glucose significantly promote the DNA content of goose hepatocytes; 5 mmol/Land 20 mmol/L glucose had no evident effect on protein content of Cyclin D1, while 35 mmol/L glucose significantly increased the protein content of Cyclin D1; relative mRNA level of Cyclin D1, Cyclin D2 and Cyclin D3 increased with the rising of glucose concentration, and the promoting effect at 35 mmol/L glucose was bigger than that of other groups. [Conclusion] In conclusion, glucose could promote the cell proliferation of goose primary hepatocytes.
韩春春、万火福、刘丹丹
细胞生物学生物化学
葡萄糖鹅原代肝细胞细胞增殖
glucosegoose primary hepatocytescell proliferation
韩春春,万火福,刘丹丹.葡萄糖对鹅原代肝细胞增殖的影响[EB/OL].(2013-10-15)[2025-08-03].http://www.paper.edu.cn/releasepaper/content/201310-148.点此复制
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