耐病和感病番茄材料中番茄黄化曲叶病毒源小RNA的特征和功能研究
haracterization and function of Tomato yellow leaf curl virus-derived small RNAs generated in tolerant and susceptible tomato varieties
与病毒抗性材料不同，耐病性植物通常允许病毒的侵染与复制，并能产生病毒源小RNA（vsRNA），这一特征对于研究比较耐病材料和感病材料间vsRNA抵抗病毒的效率十分重要。本文比较了番茄黄化曲叶病毒（Tomato yellow leaf curl virus，TYLCV）耐、感病材料在接种病毒后产生的vsRNA的各个特征。结果发现两种材料中vsRNA在病毒基因组上的分布趋势非常一致，但在含量上，耐病材料中要比感病材料的少。利用降解组测序分析了接种病毒后耐、感病材料间vsRNA介导的病毒转录本降解产物，并利用焦磷酸测序分析了病毒DNA甲基化程度，结果显示并非所有vsRNA均有介导病毒转录本剪切或病毒DNA甲基化的能力。进一步比较两类材料接种病毒不同时期其病毒DNA和mRNA的含量，结合vsRNA的特征图谱分析，认为病毒侵染后期，源于病毒的vsRNA含量与病毒转录本含量直接相关，而vsRNA介导的病毒转录本的剪切和DNA甲基化并不能完全抑制病毒的复制，且该功能在耐、感病材料之间并无特殊性。
Virus-tolerant plant, which allows the accumulation of virus and then generate virus-derived small RNAs (vsRNAs), valuable materials to reveal the antiviral efficiency of vsRNAs. Here, a comparison of vsRNAs in Tomato yellow leaf curl virus tolerant and in susceptible tomato varieties showed the consistent trend of vsRNAs' distribution on virus genome, which is presented as an obvious characteristic. However, the expression level of vsRNA in tolerant variety is less than that in susceptible variety. Slicing targets of vsRNA-mediated viral transcripts were investigated using parallel analysis of RNA ends, and geminivirus DNA methylation was determined by bisulfite sequencing, which uncovered that not all vsRNAs participated in viral mRNA degradation and DNA methylation. Additionally, by comparing with the expression pattern of vsRNAs, viral DNA and mRNA, we proposed the quantity of vsRNAs is corresponding to the expression level of viral mRNA, while the virus-suppression of vsRNAs is not high-efficient.
凌建、白描、林润茂、杨国顺、茆振川、谢丙炎、陈文婷
分子生物学植物学遗传学
番茄黄化曲叶病毒病毒介导的RNA沉默病毒源小RNA降解组
omato yellow leaf curl virusvirus-induced RNA silencingvirus-derived small RNAdegradome
凌建,白描,林润茂,杨国顺,茆振川,谢丙炎,陈文婷.耐病和感病番茄材料中番茄黄化曲叶病毒源小RNA的特征和功能研究[EB/OL].(2016-05-18)[2025-08-24].http://www.paper.edu.cn/releasepaper/content/201605-583.点此复制
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