健脾益气摄血方对ITP模型小鼠血清SIgA和β-EP影响研究
Effect of prescription JIANPIYIQISHEXUE on SIgA and β-EP in serum from mice model of ITP
目的：研究健脾益气摄血方对ITP小鼠血清中SIgA（分泌型免疫球蛋白A）、β-EP（β-内啡肽）的影响。方法：免疫造模法建立ITP小鼠模型，以强的松和健脾益气摄血方（颗粒）为干预药物，酶联免疫吸附法（ELISA）检测小鼠血清SIgA和β-EP含量。结果：①与空白对照组比较，模型小鼠血清SIgA、β-EP含量升高，具有统计学意义（p<0.05）。②与模型对照组比较，经强的松干预治疗后SIgA含量下调，有统计学意义（p<0.05）；经健脾益气摄血方干预后，β-EP含量下调，具有统计学意义（p<0.05）。结果提示，强的松对SIgA、β-EP均有调节效应；健脾益气摄血方虽然不能调控SIgA，但对β-EP具有调控作用。结论：健脾益气摄血方治疗ITP效应机制与调控β-EP含量，影响免疫问答有一定关系。
Objectives: To investigate the effect of prescription JIANPIYIQISHEXUE on SIgA and β-EP in serum from ITP mice model. Methods: The mice model of ITP was established by immune method. Prednisone and prescription JIANPIYIQISHEXUE (granules) were given, and the changes of SIgA and β-EP in serum were detected by ELISA. Results: ① The content of SIgA and β-EP in serum was up-regulated in ITP group (p<0.05). ② Compared with ITP group, the content of SIgA reduced in prednisone group (p<0.05), the content of β-EP reduced in JIANPIYIQISHEXUE group (p<0.05). The results showed SIgA and β-EP were both regulated by prednisone, and only β-EP was regulated by prescription JIANPIYIQISHEXUE. Conclusions: The mechanism of the regulation of ITP by prescription JIANPIYIQISHEXUE was related with β-EP and the immune response.
褚雨霆、陈科、郑蕾、岳立云、孙艳平、何昊、侯丽、陈信义、李天天、徐玥、秦蓓
中医学基础医学内科学
健脾益气摄血SIgAβ-EP
JIANPIYIQISHEXUESIgAβ-EP
褚雨霆,陈科,郑蕾,岳立云,孙艳平,何昊,侯丽,陈信义,李天天,徐玥,秦蓓.健脾益气摄血方对ITP模型小鼠血清SIgA和β-EP影响研究[EB/OL].(2015-06-18)[2025-08-11].http://www.paper.edu.cn/releasepaper/content/201506-244.点此复制
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