VEGF-C高表达在头颈部肿瘤细胞中的作用研究
he role of VEGF-C in head and neck squmous cell carcinoma cells
摘要；目的 探讨口腔鳞状细胞癌（OSCC）中血管内皮生长因子－C的表达与血管和淋巴管生成及其下游通路的关系，为临床靶向治疗头颈部肿瘤提供理论依据。方法 本实验拟用质粒转染技术调控VEGF-C在头颈部肿瘤细胞系HN5，MDA1986和Tu195中的表达。通过细胞体外培养，通过划痕实验和MTT生长曲线观察表达不同含量的VEGF-C的肿瘤细胞的迁移能力，并使用Western－blot实验检测AKT、MAPK等通路的变化情况。通过细胞体内成瘤实验探究在头颈部肿瘤细胞中VEGF-C对血管和淋巴管生成的作用。结果 上调VEGF-C的口腔鳞癌细胞增殖能力和迁移能力增强；AKT,STAT－1可能是其下游通路。
Objective: We investigated the relationship between the expression of vascular endothelial growth factor-C and angiogenesis , lymphangiogenesis and its downstream pathway in oral squamous cell carcinoma (OSCC) to provide a theoretical basis for the clinical treatment of head and neck tumors. Methods The expression of VEGF-C in head and neck tumor cell lines HN5, MDA1986 and Tu195 was controlled by plasmid transfection. We observed The migration ability of VEGF-C cells by scratch test and MTT growth curve. And we detected the changes of AKT and MAPK pathways by Western-blot. The effect of VEGF-C on the production of blood vessels and lymphatic vessels in head and neck tumor cells was investigated by in vivo tumor experiments. Results The proliferation and migration ability of oral squamous cell carcinoma with VEGF-C were up-regulated. AKT and STAT-1 might be the downstream pathway.
洪筠、闫一诺、程芷兰、涂叶梓、吴佳颖
肿瘤学基础医学口腔科学
VEGF-C口腔鳞状细胞癌血管淋巴管
EGF-Coral square cell carcinomablood vesselslymphatic vessels
洪筠,闫一诺,程芷兰,涂叶梓,吴佳颖.VEGF-C高表达在头颈部肿瘤细胞中的作用研究[EB/OL].(2017-05-17)[2025-06-06].http://www.paper.edu.cn/releasepaper/content/201705-1070.点此复制
评论