大蕉各种外植体及其培养物中的SERK表达

o understand the relationship between the expression of the SERK-like gene and the embryogenic competence of banana (Musa ABB，cv. Dajiao) in vitro cultures. In this study the semi-quantitative RT-PCR method was used to analyse the expression of SERK gene in the different explants which may be used in tissue culture. The explants included pseudo stem, roots, leaves, immature male flower and young fruit as well as callli induced from micro-cross section of the pseudo stem cultured in three different culture system. The results showed that a certain level expression of the SERK gene could be found in the tested roots、fruit、pseudo stem and immature flowers，however the expression level of this gene in the immature flowers was higher than that in the other tested tissues. It could be a good explanation for why the immature flowers are the best explants for embryogenic callus induction of banana in many reported papers elsewhere. Analysis of expression of the SERK gene in three calli, induced from the micro-cross section on three media, revealed that the expression level of type II callus which was the embryogenic callus was higher than that in other two kind calli. Therefore, there is closed correlation between the expression of the SERK gene and the embryogenic competence of banana callus , and the SERK gene is proved to be an efficient molecular marker for optimizing the culture condition to obtain the embryogenic cultures of banana , at least for Musa ABB，cv. Dajiao in micro-cross section culture system.