托瑞米芬对平滑肌细胞的影响研究

Objective: In this study, using vascular smooth muscle cells as cell model in vitro, aiming to investigate the effect of toremifene (TOR), a new generation of antineoplastic drugs, on biology behavior and migration mechanism of vascular smooth muscle cells (VSMCs), the effect of toremifene against restenosis (RS) was investigated. Methods: MTT method, flow cytometry method and scratch wound assay were used to examine cell growth, apoptosis and migration. Immunocytochemistry and immunoblotting were used to examine the expression of PCNA, P53 and protein of Rho/ROCK signal transmission pathway. Results: The high concentration of TOR can inhibit cell proliferation and the inhibiting effect was gradually enhanced with increasing TOR concentration; TOR can significantly promote cell apoptosis; TOR can significantly inhibit the group cell movement and migration behavior; the higher concentration of TOR treatment inhibited cell growth and induced cell apoptosis, the expression of PCNA decreased with the increasing TOR concentration; the expression of P53 increased with the increasing TOR concentration, which indicates that TOR accelerated cell apoptosis by promoting expression of apoptosis gene P53; TOR significantly inhibited the expression of Integrin β1 and Rho, and decreased the ROCK signaling pathways factor MLC and pMLC expression level. Conclusion：Our results indicated that TOR may inhibit cell growth and induce cell apoptosis and inhibit cell migration behavior by the Integrin β1- Rho- ROCK- (p) MLC signaling pathway.??????????