力学刺激对口腔鳞癌细胞caspase-3表达的影响
Effects of Mechanical stimulation on caspase-3 expression of Human oral Squamous Cell Carcinoma Cells
目的：探讨力学刺激对体外培养口腔鳞状细胞癌KB细胞的凋亡基因caspase-3表达的变化。方法：采用FX-4000柔性基底加载系统对人口腔鳞状细胞癌KB细胞进行体外加载培养，卸载后运用流式细胞仪检测分析KB细胞的凋亡率，通过实时荧光定量PCR检测分析凋亡基因caspase-3表达的变化。结果：在加载程式（方波，幅度15%，频率0.5Hz，时间24h）的拉伸培养下，KB细胞的caspase-3 mRNA水平高于正常对照组，差异具有统计学意义（P<0.05）。结论：力学刺激能够诱导凋亡信号通路中的caspase家族产生级联反应，通过激活caspase-3因子而诱导人口腔鳞状细胞癌KB细胞发生凋亡。
Objective：To study the effects of Mechanical stimulation on caspase-3 protein expression of Human oral Squamous Cell Carcinoma KB Cells in vitro. Method: The Human oral Squamous Cell Carcinoma KB Cells was cultured by FX-4000 Strain unit, The apoptotic rate was detected by flow cytometry, The protein expression of caspase-3 was examined by Real-time fluorogenic quantitative polymerase chain reaction (FQ-PCR). Result: The protein expression of caspase-3 increased significantly compared with control after the KB cells were subjected to regimens(square wave，15% elongation amplitude，0. 5Hz，24h ) , the difference had statistical significance( P < 0. 05). Conclusion: Mechanical stimulation activated caspase-3 which induced the KB cell apoptosis, therefore caspase-3 is involved in the mechanical stimulation-induced cell apoptosis.
高鹏、郝凤翔、曲华
基础医学肿瘤学口腔科学
力学刺激caspase-3KB细胞凋亡率
Mechanical stimulationcaspase-3KB cellsapoptosis
高鹏,郝凤翔,曲华.力学刺激对口腔鳞癌细胞caspase-3表达的影响[EB/OL].(2012-02-22)[2025-07-21].http://www.paper.edu.cn/releasepaper/content/201202-825.点此复制
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