芦荟大黄素影响巨噬细胞升高[Ca2+]i和释放TNF-α的作用特征
EFFECTS OF ALOE-EMODIN ON INCREASE IN [Ca2+]i AND TNF-α PRODUCTION IN RAT PERITONEAL MACROPHAGES
本文应用MTT法检测肿瘤坏死因子（TNF-α）量，同时利用细胞钙离子成像分析系统检测单细胞内自由钙离子浓度（[Ca2+]i）的动态变化，来研究芦荟大黄素（Aloe-emodin）对正常的和细菌脂多糖 (LPS）刺激的大鼠腹腔巨噬细胞（PMφ）释放TNF-α和[Ca2+]i的影响。结果显示芦荟大黄素可较强活化正常PMφ释放TNF-α，同时诱发正常PMφ [Ca2+]i呈振荡式变化，[Ca2+]i变化主要来源于胞外钙内流。药物升高细胞[Ca2+]i和促进TNF-α释放都随其浓度增加而增强。芦荟大黄素对LPS刺激的PMφ的影响有较复杂的剂量依赖性特征：即低浓度芦荟大黄素（10-7mol/L、10-6mol/L）抑制LPS诱发的[Ca2+]i升高和TNF-α的释放；10-5mol/L的芦荟大黄素则对LPS诱发的[Ca2+]i峰及TNF-α释放量没有明显影响。以上结果说明，芦荟大黄素对PMφ释放TNF-α和升高[Ca2+]i表现出双向调节作用，但其活化正常PMφ和抑制LPS的刺激作用有相反的量-效关系。同时芦荟大黄素对PMφ的[Ca2+]i动力学的调制，是其调节细胞释放TNF-α的信号传导通路中的重要环节。
y using MTT assay and image analysis system of cytosolic free calcium（[Ca2+]i） in single cell, the characteristics and mechanisms of Aloe-emodin on [Ca2+]i dynamics and TNF-α production in normal and LPS-stimulated rat peritoneal macrophages (PMφ) were investigated. Aloe-emodin can increase TNF-α production and [Ca2+]i of normal PMφ in a dose-dependent manner: the more Aloe-emodin is, the more TNF-α releases and increase [Ca2+]i . Furthermore, Aloe-emodin causes calcium oscillation in normal PMφ, the medicine induce cytosolic calcium increase mainly by calcium influx from extracellular. Additionally, Aloe-emodin show complex effects in a dose-dependent manner on PMφ due to LPS. At low concentration, Aloe-emodin can inhibit the TNF-α production due to LPS and blunt the transient increase in [Ca2+]i induced by LPS. But, 10-5mol/L Aloe-emodin has not a distinct effect on TNF-α production due to LPS and the transient increase in [Ca2+]i induced by LPS. These results indicate that the bidi
胡芬、刘慧君、张莉平、杨文修、陈立君
基础医学生物化学生理学
芦荟大黄素巨噬细胞脂多糖[Ca2+]iNF-α
Aloe-emodinmacrophagesLipopolysaccharidea2+]i TNF-α
胡芬,刘慧君,张莉平,杨文修,陈立君.芦荟大黄素影响巨噬细胞升高[Ca2+]i和释放TNF-α的作用特征[EB/OL].(2005-07-11)[2025-09-02].http://www.paper.edu.cn/releasepaper/content/200507-84.点此复制
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