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深低温停循环对大鼠海马神经元线粒体超微结构的影响和定量分析

Quantative Analysis and Ultrastructural Observation of Mitochondria in Hippocampal Neurons of Rats under Whole Body Deep Hypothermic Circulatory Arrest

中文摘要英文摘要

目的 观察深低温停循环后大鼠海马神经元线粒体的超微结构改变,同时对超微结构的变化进行定量分析。方法 取SD大鼠40只,随机分为3组:深低温停循环(DHCA)组、常温停循环(NTCA)组和正常对照(NC)组(N=12)。采用体外插管法建立大鼠闭胸式体外循环模型,DHCA组体外循环建立后在深低温(<20℃)条件下停循环10分钟;NTCA组在正常温度(36.8℃~37℃)条件下停循环10分钟;NC组建立体外循环后不进行降温和停循环处理,维持正常温度(36.8℃~37℃)体外循环10分钟。之后迅速断头取海马,制成透射电镜标本,观察线粒体超微结构并进行体视学分析,结果采用SPSS11.5软件进行统计学分析。结果 线粒体周长、直径、平均灰度及三维参数Vv、Nv、V、δ在NTCA组分别与NC组和DHCA组比较有统计学差异(P<0.05)。结论 DHCA可以保持海马线粒体二维和三维参数的多个指标相对稳定,减轻线粒体肿胀程度,促进能量代谢。

Objective To investigate the effects of deep hypothermic circulatory arrest at subcellular level by quantitative analysis and ultrastructural observation on hippocampal mitochondrion of rats.Methods Forty Sprague-Dawley (SD) rats were divided randomly into three groups: deep hypothermic circulatory arrest (DHCA) group, normal temperature circulatory arrest (NTCA) group and normal control (NC) group(n=12). The animal model of closed-chest cardiopulmonary bypass (CPB) was established in SD rats by means of external cannulation. In DHCA group,circulatory arrest of 10min were performed in the deep hypothermia (<20℃); in NTCA group, the same operation were performed in normal temperature(36.8℃~37℃)and without circulatory arrest in normal temperature(36.8℃~37℃)in NC group. And then the hippocampus were taken out of from the rats brain and were observed by electro microscope.There were some indexes were emphasized on the ultrastructure of the mitochondria of hippocampus, such as the circumference, the diameter, the mean gray scale, the volume density (Vv), the numerical density (Nv), the mean volume and the specific surface (δ) of mitochondria. Data were analyzed by software SPSS. Results There were significant difference between NTCA and DHCA /NC in parameters of circumference, diameter、mean gray scale,Vv, Nv, V and δ(P<0.05).Conclusion The technique of DHCA can stable many two-dimensional and three-dimensional indexes in hippocampal mitochondria, reduce the degree of mitochondria edema and promote energy metabolism.

高永军、徐蔚、陈飞雄、方绍龙、赵金喜、曹毅、李旭辉

基础医学生理学神经病学、精神病学

海马深低温停循环线粒体超微结构定量分析

HippocampusDHCAMitochondriaultrastructureQuantative analysis

高永军,徐蔚,陈飞雄,方绍龙,赵金喜,曹毅,李旭辉.深低温停循环对大鼠海马神经元线粒体超微结构的影响和定量分析[EB/OL].(2012-12-20)[2025-08-19].http://www.paper.edu.cn/releasepaper/content/201212-517.点此复制

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