活血利水法对兔外伤性玻璃体视网膜病变增殖膜中转化生长因子信使核糖核酸表达的影响

Objective: To study the curative and preventive effects and mechanism of HuoXueLiShui Method (HXLSM) on the effects of EGFmRNA’s expression on ERM of Traumatic Proliferative Vitreoretinopathy. Methods: 32 rabbits were selected randomly from 40 healthy adult color rabbits and induced to traumatic proliferative vitreoretinopathy models, then divided into four groups: the first group was treated with San Xue Ming Mu Tablet-Huo Xue Li Shui (HXLS) group, the second treated with Zhi Xue Qu Yu Tablet-Huo Xue Hua Yu (HXHY) group, the third treated with Zhu Ling San-Li Shui Ming Mu (LSMM) group and the model group. The rest 8 formed the blank control group.After the rabbits had been fed and administrated for a month, we observed the visibility of fundus, the degree of PVR and pathologic changes, measured EGFmRNA in epiretinal membrane(ERM) expression were observed with immunohistochemistry technique. Results: Compared with model group, the positive level of EGFmRNA, was decreased significantly in HXLS group (p<0.01). Compared with model group, the positive level of EGFmRNA decreased much in HXHY and LSMM group(p<0.05). And the level of EGFmRNA in HXLS group were lower than those in HXHY and LSMM group. The positive level of EGFmRNA in HXLS group was a little higher than those in blank group (p<0.05). The PVR in HXLS group was lighter than that in model group、HXHY and LSMM group. Conclusions: Huo Xue Hua Yu Method is the coordination of Huo Xue Hua Yu method and Shui Ming Mu method. It could inhibit and cure the formation and development of traumatic PVR by counteracting the expression of EGFmRNA on ECM as to inhibiting proliferrative cell’s hyperplasy.