基于氧化应激途径研究纳米材料二氧化钛生物安全性
Study on the biological safety of TiO2 nanoparticles based on the oxidative stress pathway
目的：通过研究纳米材料Tio2对处于氧化应激状态的小鼠的影响，探索Tio2纳米材料的生物安全性。方法：基于氧化应激途径研究纳米材料Tio2生物安全性。将100只小鼠随机分为10组，5个组为一组，分别为高、中、低浓度实验组和阴性、阳性对照组，两组分别进行尾静脉注射染毒和气管注射染毒实验。观察小鼠反应症状，同时取血清和肺组织匀浆，测定血清和肺组织中SOD活力和MDA的含量。根据过敏反应评分，确定氧化反应程度，综合实验数据判定小鼠的损伤程度。结果：随着纳米材料Tio2的浓度升高，小鼠血清和肺组织MDA含量均呈上升趋势以及SOD的活性降低，显示小鼠体内呈现较强的氧化应激状态。结论：直接暴露途经下的高浓度Tio2纳米材料对生物体具有较强的危害性。。
Objective: To study the effects of TiO2 nanoparticles in mice and explore the biological safety of nano materials TiO2. Methods: Research the biological safety of the TiO2 nanoparticles based on oxidative stress pathway research. 100 mices were randomly divided into 10 groups, the 5 group is respectively a group. Low concentration experimental group and negative, positive control group, the two groups were intravenous injection and tracheal injection exposure experiment. Mice were observed symptoms and got serum and lung homogenate for activity of SOD and content of MDA in serum and lung tissue at the same time. According to the scores of allergic reaction determine anti oxidation and degree of comprehensive experimental data determine the degree of injury in mice. Results: With the increase of TiO2 concentration of nano materials, the content of MDA in serum and lung tissue of mice was decreased and the activity of SOD increased which showed that mice showed oxidative stress status in vivo. Conclusion: The strong direct exposure of high concentration of TiO2 nanoparticles had the harm strong to the organism.
冉棋、穆亚娟、张国伟、霍树营、李斯琪、李林俸、佟小芳、张兰兰、朱新宇
环境生物学生物科学研究方法、生物科学研究技术生物化学
基础医学，纳米材料io2氧化应激生物安全性
Foundation medicineiO2NanoparticlesOxidative stressBiosafety nanomaterials
冉棋,穆亚娟,张国伟,霍树营,李斯琪,李林俸,佟小芳,张兰兰,朱新宇.基于氧化应激途径研究纳米材料二氧化钛生物安全性[EB/OL].(2016-10-28)[2025-08-11].http://www.paper.edu.cn/releasepaper/content/201610-221.点此复制
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